Director, Leonard Dobens
306B SCB
(816)235-6272
dobensl@umkc.edu

Capabilities:

• DNA sequencing of dsDNA, ssDNA and purified PCR products
• Real-time PCR

Equipment:

• ABI 7500 Real-time PCR
• ABI 3100 Genetic Analyzer from Applied Biosystems, 50 cm x 16 capillary array
• Typhoon 9400 Variable Mode Imager for phosphorimaging, chemiluminescence and fluorescence
• Storm 840 Imager by GE Healthcare for phosphorimaging and fluorescence
• Kodak Gel Logic 200 Imaging System

Director, Andrew Keightley
405C SCB
(816)235-2565
keightleyj@umkc.edu

Capabilities and services:

• In-gel trypsin digestion, and FASP (filter aided sample preparation-samples in solution)
• Protein identification by LC-tandem MS
• Whole peptidome analysis using multi-dimensional liquid chromatography (mudpit)
• Tandem Mass Tag-based quantitation/differential expression analysis
• Database searches using Proteome Discoverer and Mascot

Equipment:

• Q Exactive Plus High Resolution Mass Spectrometer (HRMS)
• Thermo Finnigan LTQ linear ion trap
• Eksigent binary nano-LC pump system (1D nanoLC)
• Eksigent quaternary nano-LC pump system (2D nanoLC)
• CTC PAL robot for fraction collection (1st dimension for mudpit), 96 well format
• Dell Xeon 3.7 processor, 36Gb memory, 400Gb SSD boot, spinning 2 x 4Tb archives

Sample Submission Guide, Sample Form and Fees

• Sample Preparation Guidelines (PDF)
• Sample Submission Form (PDF)
• HRMS Facility Fees 2019  (PDF)
• MASCOT Search Result Interpretation Guide (PDF)
• Quantitative Search Interpretation Guide (PDF)
• Proteomics Resources and Pathways Gene Ontology Analysis (PDF)
• Supported Publications (PDF)

Acting Director, Theodore White
198 BSB
(816)235-2538
whitetc@umkc.edu

Capabilities:

• Data collection for academic researchers
• NMR data analysis training by arrangement
• NMR data collection training for frequent users

Equipment:

• Oxford AS 600/51 14.1 Tesla actively shielded magnet
• {HCN} cryo and {HCX} RT probes
• Four full channels with observe, pulse and decouple functions
• Two Sun Blade 1000 and three Linux-based computers; an additional Linux computer is available within SBS

Director, Marilyn D. Yoder
526 SCB
(816)235-1986
yoderm@umkc.edu

Capabilities:

• Crystallization screens
• X-ray data collection

Equipment:

• UMKC is part of the Southeast Regional Collaborative Access Team operating two beamlines at the Advanced Proton Source at Argonne National Laboratories

Director, Edward Gogol
192 BSB
(816)235-2584
gogole@umkc.edu

Capabilities:

• Visualization of macromolecules, viruses and bacteria by negative staining

Equipment:

• JEOL 1200-EX TEM equipped with goniometric specimen stage

Director, Tara Allen
275F BSB
(816)235-2568
allent@umkc.edu

Capabilities:

• Determination of cell size (Forward Scatter – FSC) and cell complexity (Side Scatter – SSC)
• Measurement of fluorescent intensity (FL1, FL2, FL3) to assess cell viability, apoptosis, total DNA content, protein expression, cell surface or intracellular antigen presence, intracellular calcium, etc.
• Cell identification and sorting/isolating a subpopulation of cells from the sample

Equipment:

• Becton Dickinson FACSCalibur
• FlowJo Software for analysis of cytometric data

Resources:

• Flow cytometry guide by abcam (PDF)
• Introduction to Flow Cytometry and Flow Cytometry Data Analysis by Invitrogen

Director, Leonard Dobens
306B SCB
(816)235-6272
dobensl@umkc.edu

Capabilities:

• Circular-dichroism
• Surface plasmon resonance
• Analytical ultracentrifugation
• Microcalorimetry

Equipment:

• Jasco model J-720 spectropolarimeter – circular dichroism (CD)
• Beckman X-LA analytical ultracentrifuge
• PTI photon counting fluorometer with fluorescence lifetime and depolarization capabilities
• FLUOstar Plate reader
• MicroCal VP-ITC
• Dynapro dynamic light scattering instrument, with temperature control
• Biacore X

Director, Jeffrey Price
406B SCB
(816)235-2572
pricejl@umkc.edu

Capabilities:

• Total internal reflectance fluorescence (TIRF) microscopy
• Optical sectioning by confocal laser scanning (LSM) and structured illumination (SIM) microscopy
• 3D image reconstruction by deconvolution optical microscopy

Equipment:

• Nikon TIRF inverted microscope
• Olympus DeltaVision inverted microscope
• Nikon OptiGRID SIM upright microscope
• Zeiss LSM-410 inverted microscope
• Zeiss LSM-5 Pascal upright microscope
• Olympus LSM upright microscope