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Biological and Chemical Sciences

Biological and Chemical Sciences

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Each of our facilities includes state-of-the-art equipment and skilled technical staff to aid with biological and chemical research.

Facilities Information

Director, Leonard Dobens
306B SCB
(816)235-6272
dobensl@umkc.edu

Capabilities:

  • DNA sequencing of dsDNA, ssDNA and purified PCR products
  • Real-time PCR

Equipment:

  • ABI 7500 Real-time PCR
  • ABI 3100 Genetic Analyzer from Applied Biosystems, 50 cm x 16 capillary array
  • Typhoon 9400 Variable Mode Imager for phosphorimaging, chemiluminescence and fluorescence
  • Storm 840 Imager by GE Healthcare for phosphorimaging and fluorescence
  • Kodak Gel Logic 200 Imaging System

Director, Andrew Keightley
405C SCB
(816)235-2565
keightleyj@umkc.edu

Capabilities and services:

  • In-gel trypsin digestion, and FASP (filter aided sample preparation-samples in solution)
  • Protein identification by LC-tandem MS
  • Whole peptidome analysis using multi-dimensional liquid chromatography (mudpit)
  • Tandem Mass Tag-based quantitation/differential expression analysis
  • Database searches using Proteome Discoverer and Mascot

Equipment:

  • Q Exactive Plus High Resolution Mass Spectrometer (HRMS)
  • Thermo Finnigan LTQ linear ion trap
  • Eksigent binary nano-LC pump system (1D nanoLC)
  • Eksigent quaternary nano-LC pump system (2D nanoLC)
  • CTC PAL robot for fraction collection (1st dimension for mudpit), 96 well format
  • Dell Xeon 3.7 processor, 36Gb memory, 400Gb SSD boot, spinning 2 x 4Tb archives

Sample Submission Guide, Sample Form and Fees

Acting Director, Theodore White
198 BSB
(816)235-2538
whitetc@umkc.edu

Capabilities:

  • Data collection for academic researchers
  • NMR data analysis training by arrangement
  • NMR data collection training for frequent users

Equipment:

  • Oxford AS 600/51 14.1 Tesla actively shielded magnet
  • {HCN} cryo and {HCX} RT probes
  • Four full channels with observe, pulse and decouple functions
  • Two Sun Blade 1000 and three Linux-based computers; an additional Linux computer is available within SBS

Director, Marilyn D. Yoder
526 SCB
(816)235-1986
yoderm@umkc.edu

Capabilities:

  • Crystallization screens
  • X-ray data collection

Equipment:

  • UMKC is part of the Southeast Regional Collaborative Access Team operating two beamlines at the Advanced Proton Source at Argonne National Laboratories

Director, Edward Gogol
192 BSB
(816)235-2584
gogole@umkc.edu

Capabilities:

  • Visualization of macromolecules, viruses and bacteria by negative staining

Equipment:

  • JEOL 1200-EX TEM equipped with goniometric specimen stage

Director, Tara Allen
275F BSB
(816)235-2568
allent@umkc.edu

Capabilities:

  • Determination of cell size (Forward Scatter – FSC) and cell complexity (Side Scatter – SSC)
  • Measurement of fluorescent intensity (FL1, FL2, FL3) to assess cell viability, apoptosis, total DNA content, protein expression, cell surface or intracellular antigen presence, intracellular calcium, etc.
  • Cell identification and sorting/isolating a subpopulation of cells from the sample

Equipment:

  • Becton Dickinson FACSCalibur
  • FlowJo Software for analysis of cytometric data

Resources:

  • Flow cytometry guide by abcam (PDF)
  • Introduction to Flow Cytometry and Flow Cytometry Data Analysis by Invitrogen

Director, Leonard Dobens
306B SCB
(816)235-6272
dobensl@umkc.edu

Capabilities:

  • Circular-dichroism
  • Surface plasmon resonance
  • Analytical ultracentrifugation
  • Microcalorimetry

Equipment:

  • Jasco model J-720 spectropolarimeter – circular dichroism (CD)
  • Beckman X-LA analytical ultracentrifuge
  • PTI photon counting fluorometer with fluorescence lifetime and depolarization capabilities
  • FLUOstar Plate reader
  • MicroCal VP-ITC
  • Dynapro dynamic light scattering instrument, with temperature control
  • Biacore X

Director, Jeffrey Price
406B SCB
(816)235-2572
pricejl@umkc.edu

Capabilities:

  • Total internal reflectance fluorescence (TIRF) microscopy
  • Optical sectioning by confocal laser scanning (LSM) and structured illumination (SIM) microscopy
  • 3D image reconstruction by deconvolution optical microscopy

Equipment:

  • Nikon TIRF inverted microscope
  • Olympus DeltaVision inverted microscope
  • Nikon OptiGRID SIM upright microscope
  • Zeiss LSM-410 inverted microscope
  • Zeiss LSM-5 Pascal upright microscope
  • Olympus LSM upright microscope